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| Barbier, Ewa; Lagorce, Arnaud; Hachemi, Amine; Dutertre, Murielle; Gorlas, Aurore; Morand, Lucie; Saint-pierre, Christine; Ravanat, Jean-luc; Douki, Thierry; Armengaud, Jean; Gasparutto, Didier; Confalonieri, Fabrice; Breton, Jean. |
| The hyperthermophilic archaeon Thermococcus gammatolerans can resist huge doses of γ-irradiation – up to 5.0 kGy – without loss of viability. The potential to withstand such harsh conditions is probably due to complementary passive and active mechanisms, including repair of damaged chromosomes. In this work, we documented the formation and repair of oxidative DNA lesions in T. gammatolerans. The basal level of the oxidized nucleoside, 8-oxo-2′-deoxyguanosine (8-oxo-dGuo), was established at 9.2 (+/- 0.9) 8-oxo-dGuo per 106 nucleosides, a higher level than those usually measured in eukaryotic cells or bacteria. A significant increase in oxidative damage, i.e. up to 24.2 (+/- 8.0) 8-oxo-dGuo/106 nucleosides, was measured for T .gammatolerans exposed to a 5.0... |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00353/46388/46015.pdf |
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| Rubio, Tristan; Oyanedel, Daniel; Labreuche, Yannick; Toulza, Eve; Luo, Xing; Bruto, Maxime; Chaparro, Cristian; Torres, Marta; De Lorgeril, Julien; Haffner, Philippe; Vidal-dupiol, Jeremie; Lagorce, Arnaud; Petton, Bruno; Mitta, Guillaume; Jacq, Annick; Le Roux, Frederique; Charriere, Guillaume; Destoumieux-garzon, Delphine. |
| Vibrio species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How Vibrio subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent Vibrio species in an ecologically relevant host model, oyster, to study interactions with marine Vibrio species. All Vibrio strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional... |
| Tipo: Text |
Palavras-chave: T6SS; Toxin; Dual RNA-seq; Cytolysis; Pathogenesis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00507/61837/65900.pdf |
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